James Arnold Taylor

Character: Leonardo (voice)

Mitchell Whitfield

Character: Donatello / Additional Voices (voice)

Nolan North

Character: Raphael / Nightwatcher (voice)

Mikey Kelley

Character: Michelangelo (voice)

Sarah Michelle Gellar

Character: April O'Neil (voice)

Chris Evans

Character: Casey Jones (voice)

Mako

Character: Splinter (voice)

Patrick Stewart

Character: Winters (voice)

Kevin Smith

Character: Diner Cook (voice)

Laurence Fishburne

Character: Narrator (voice)

Zhang Ziyi

Character: Karai (voice)

John DiMaggio

Character: Colonel Santino (voice)

Paula Mattioli

Character: General Serpiente (voice)

Kevin Michael Richardson

Character: General Aguila / Additional Voices (voice)

Fred Tatasciore

Character: General Gato / Additional Voices (voice)

Dee Bradley Baker

Character: Additional Voices (voice)

Greg Baldwin

Character: Splinter (voice)

Jeff Bennett

Character: Additional Voices (voice)

Jim Cummings

Character: Additional Voices (voice)

Grey DeLisle

Character: Additional Voices (voice)

Chris Edgerly

Character: Additional Voices (voice)

Kim Mai Guest

Character: Additional Voices (voice)

Jennifer Hale

Character: Additional Voices (voice)

Jess Harnell

Character: Additional Voices (voice)

Phil LaMarr

Character: Additional Voices (voice)

Paul Michael Robinson

Character: Additional Voices (voice)

Tara Strong

Character: Additional Voices (voice)

Billy West

Character: Additional Voices (voice)

Kevin Munroe

Character: Diner Patron (voice) (uncredited)

Frank Welker

Character: General Mono (voice) (uncredited)