Cedric Hardwicke

Character: Dr. Ludwig Frankenstein / Dr. Henry Frankenstein (ghostly image)

Ralph Bellamy

Character: Erik Ernst

Lionel Atwill

Character: Dr. Bohmer

Bela Lugosi

Character: Ygor

Evelyn Ankers

Character: Elsa Frankenstein

Lon Chaney Jr.

Character: The Frankenstein Monster

Janet Ann Gallow

Character: Cloestine Hussman

Barton Yarborough

Character: Dr. Kettering

Doris Lloyd

Character: Martha the Housekeeper

Leyland Hodgson

Character: Chief Constable

Olaf Hytten

Character: Adolph Hussman

Holmes Herbert

Character: Magistrate

Richard Alexander

Character: Villager at Council Meeting (uncredited)

Lionel Belmore

Character: Councilman (uncredited)

Chet Brandenburg

Character: Villager (uncredited)

Colin Clive

Character: Dr. Henry Frankenstein (flashback) (uncredited)

Harry Cording

Character: Mob Leader (uncredited)

George Eldredge

Character: Constable (uncredited)

Dwight Frye

Character: Villager at Meeting / Grave Robber (flashback) (uncredited)

Lawrence Grant

Character: Mayor (uncredited)

Otto Hoffman

Character: Villager (uncredited)

Brandon Hurst

Character: Hans, Villager at Council Meeting (uncredited)

Michael Mark

Character: Councilman (uncredited)

Jimmy Phillips

Character: Boy Playing Indian (uncredited)

William Smith

Character: Boy (uncredited)

Ernie Stanton

Character: Constable (uncredited)

Julius Tannen

Character: Sektal (uncredited)

Harry Tenbrook

Character: Villager at Hearing (uncredited)

Glen Walters

Character: Mother of Hungry Children (uncredited)

Janet Warren

Character: Goose-herd (uncredited)

Teddy Infuhr

Character: Boy (uncredited)