Shameik Moore

Character: Miles Morales (voice)

Jake Johnson

Character: Peter B. Parker (voice)

Hailee Steinfeld

Character: Gwen Stacy (voice)

Mahershala Ali

Character: Uncle Aaron (voice)

Brian Tyree Henry

Character: Jefferson Davis (voice)

Lily Tomlin

Character: Aunt May (voice)

Luna Lauren Velez

Character: Rio Morales (voice)

Zoë Kravitz

Character: Mary Jane (voice)

John Mulaney

Character: Spider-Ham (voice)

Kimiko Glenn

Character: Peni Parker (voice)

Nicolas Cage

Character: Spider-Man Noir (voice)

Kathryn Hahn

Character: Doc Ock (voice)

Liev Schreiber

Character: Wilson Fisk (voice)

Chris Pine

Character: Peter Parker (voice)

Natalie Morales

Character: Miss Calleros (voice)

Edwin H. Bravo

Character: Brooklyn Visions Security Guard (voice)

Oscar Isaac

Character: Interesting Person #1 (voice)

Greta Lee

Character: Interesting Person #2 (voice)

Stan Lee

Character: Stan (voice)

Jorma Taccone

Character: Green Goblin / Last Dude (voice)

Joaquín Cosío

Character: Scorpion (voice)

Marvin Jones III

Character: Tombstone (voice)

Kim Yarbrough

Character: Scientist in Cafeteria (voice)

Lake Bell

Character: Vanessa Fisk (voice)

Jessica Mikayla Adams

Character: Brooklyn Friend (voice)

Gredel Berrios Calladine

Character: Brooklyn Friend (voice)

Sarah D. Cole

Character: Brooklyn Friend (voice)

Kelby Joseph

Character: Brooklyn Friend (voice)

Mimi Davila

Character: Brooklyn Visions Student (voice)

Claudia Choi

Character: Brooklyn Visions Teacher (voice)

Melanie Haynes

Character: Brooklyn Visions Teacher (voice)

Joseph Izzo

Character: Brooklyn Visions Teacher (voice)

Nick Jaine

Character: Brooklyn Visions Teacher (voice)

Muneeb Rehman

Character: Brooklyn Visions Teacher (voice)

Carlos Zaragoza

Character: Brooklyn Visions Teacher (voice)

Post Malone

Character: Brooklyn Bystander (voice)

David Applebee

Character: Additional Voices (voice)

Juan Carlos Arvelo

Character: Additional Voices (voice)

Adam Brown

Character: Additional Voices (voice)

Jon Bruno

Character: Additional Voices (voice)

Darcy Rose Byrnes

Character: Additional Voices (voice)

Oscar Camacho

Character: Additional Voices (voice)

June Christopher

Character: Additional Voices (voice)

Alycia Cooper

Character: Additional Voices (voice)

Michelle Jubilee Gonzalez

Character: Additional Voices (voice)

Terrence Hardy Jr.

Character: Additional Voices (voice)

Bridget Hoffman

Character: Additional Voices (voice)

Rif Hutton

Character: Additional Voices (voice)

Miguel Jiron

Character: Additional Voices (voice)

Harrison Knight

Character: Additional Voices (voice)

Lex Lang

Character: Additional Voices (voice)

Donna Lynn Leavy

Character: Additional Voices (voice)

Andrew Leviton

Character: Additional Voices (voice)

Caitlin McKenna

Character: Additional Voices (voice)

Scott Menville

Character: Additional Voices (voice)

Christopher Miller

Character: Additional Voices (voice)

Arthur Ortiz

Character: Additional Voices (voice)

Juan Pacheco

Character: Additional Voices (voice)

Devika Parikh

Character: Additional Voices (voice)

Shakira Ja'nai Paye

Character: Additional Voices (voice)

Courtney Peldon

Character: Additional Voices (voice)

Chrystee Pharris

Character: Additional Voices (voice)

Jacqueline Pinol

Character: Additional Voices (voice)

Juan Pope

Character: Additional Voices (voice)

Al Rodrigo

Character: Additional Voices (voice)

Joseph Sanfelippo

Character: Additional Voices (voice)

Justin Shenkarow

Character: Additional Voices (voice)

Dennis Singletary

Character: Additional Voices (voice)

Warren Sroka

Character: Additional Voices (voice)

Melissa Sturm

Character: Additional Voices (voice)

Holly Walker

Character: Additional Voices (voice)

Jason Linere-White

Character: Additional Voices (voice)

Ruth Zalduondo

Character: Additional Voices (voice)