Saoirse Ronan

Character: Mary Stuart

Margot Robbie

Character: Queen Elizabeth I

Jack Lowden

Character: Lord Darnley

David Tennant

Character: John Knox

Guy Pearce

Character: William Cecil

Gemma Chan

Character: Bess of Hardwick

James McArdle

Character: James, Earl of Moray

Ismael Cruz Cordova

Character: David Rizzio

Martin Compston

Character: Earl of Bothwell

Joe Alwyn

Character: Robert Dudley

Brendan Coyle

Character: Earl of Lennox

Ian Hart

Character: Lord Maitland

Richard Cant

Character: Thomas Andrews

Guy Rhys

Character: Earl of Kent

Thom Petty

Character: Earl of Shrewsbury

Izuka Hoyle

Character: Mary Seton

John Ramm

Character: Bull

Simon Russell Beale

Character: Robert Beale

Maria Dragus

Character: Mary Fleming

Liah O'Prey

Character: Mary Livingston

Eileen O'Higgins

Character: Mary Beaton

Greg Miller Burns

Character: Thomas Hepburn

Aneurin Pascoe

Character: John Hepburn

Adrian Derrick-Palmer

Character: George Dalgleish

Kal Sabir

Character: The Laird of Ramorny

Luke Kidd

Character: James Haliburton

Adrian Lester

Character: Lord Randolph

Georgia Burnell

Character: Kate Carey

Alan Turkington

Character: Thomas Jenye

Euan Macnaughton

Character: William Kirkcaldy of Grange

Scot Greenan

Character: Hector Maclean

Karen Dunbar

Character: Commoner

Ian Hallard

Character: Sir Richard Knightley

Andrew Rothney

Character: King James I

Angela Bain

Character: Snuffer Woman

Abby Cassidy

Character: Fotheringhay Gentlewoman

Shalisha James-Davis

Character: Fotheringhay Gentlewoman

Adam Bond

Character: Sir William Douglas

Katharine O'Donnelly

Character: Young Holyrood Servant

Grace Molony

Character: Dorothy Stafford

Luke Hobson

Character: Henry Killigrew

Ben Wiggins

Character: John Tamworth

Eldredd Wolf

Character: William Killigrew

Eric MacLennan

Character: Portrait Painter

Nathen East

Character: Andrew Ker of Fawdonside

Daniel Booroff

Character: Knox's Secretary

Sean Buchanan

Character: Francis Yaxley

Matthew Pidgeon

Character: Wedding Announcer

Benjamin Barraclough

Character: Officer

Jordan Turk

Character: James MacDonald

Adam Stevenson

Character: Urie Campbell

Chris Grahamson

Character: Moray's Drummer

Ed Jones

Character: William Taylor

Owen Gorman

Character: Bishop

John Stahl

Character: Fisherman

Kadiff Kirwan

Character: Attaché

Alex Beckett

Character: Sir Walter Mildmay

Will Rowlands

Character: Soldier

Grant Crookes

Character: Bothwell

Philip Gascoyne

Character: English Nobleman (uncredited)