Megalyn Echikunwoke

Character: Mari McCabe / Vixen (voice)

Stephen Amell

Character: Oliver Queen / Green Arrow (voice)

Grant Gustin

Character: Barry Allen / The Flash (voice)

Emily Bett Rickards

Character: Felicity Smoak / Overwatch (voice)

Carlos Valdes

Character: Cisco Ramon (voice)

Victor Garber

Character: Dr. Martin Stein / Firestorm (voice)

Brandon Routh

Character: Ray Palmer / The Atom (voice)

Katie Cassidy

Character: Laurel Lance / Black Canary (voice)

María Canals-Barrera

Character: Dr. Vargas (voice)

Trevor Devall

Character: Drake / Jones (voice)

Neil Flynn

Character: Chuck (voice)

Hakeem Kae-Kazim

Character: Eshu (voice)

Brandon Keener

Character: Silk Tie (voice)

Toks Olagundoye

Character: Mari's Mother / News Reporter / Teaching Assistant (voice)

Anika Noni Rose

Character: Kuasa (voice)

Sean Patrick Thomas

Character: Professor Macalester (voice)

Kari Wuhrer

Character: Patty (voice)

Kevin Michael Richardson

Character: D.O.C Guard (voice)

Kimberly Brooks

Character: Young Mari (voice)