Fredric March

Character: Marcus Superbus - Prefect of Rome

Elissa Landi

Character: Mercia

Claudette Colbert

Character: Empress Poppaea

Charles Laughton

Character: Emperor Nero Claudius Caesar

Ian Keith

Character: Tigellinus

Arthur Hohl

Character: Titus

Harry Beresford

Character: Favius Fontelas

Tommy Conlon

Character: Stephan

Ferdinand Gottschalk

Character: Glabrio

Vivian Tobin

Character: Dacia

William V. Mong

Character: Licinius

Joyzelle Joyner

Character: Ancaria

Richard Alexander

Character: Viturius

Nat Pendleton

Character: Strabo

Clarence Burton

Character: Servillius

Harold Healy

Character: Tybul

Robert Seiter

Character: Philodemus

Charles Middleton

Character: Tyros

Henry Brandon

Character: Colosseum Spectator (uncredited)

John Carradine

Character: Christian Martyr / Gladiator Leader / Voice in Coliseum Mob (uncredited)

Dave O'Brien

Character: Christian on Stairway (uncredited)

Sally Rand

Character: Crocodiles' Victim (uncredited)

Arthur Shields

Character: Chaplain Costello (1944 Re-Release Prologue) (uncredited)

Tom Tully

Character: Hoboken (1944 Re-Release Prologue) (uncredited)

Lionel Belmore

Character: Bettor of 300 Silver

Ynez Seabury

Character: Little Girl