Frank Welker

Character: Scooby-Doo / Fred Jones (voice)

Grey DeLisle

Character: Daphne Blake (voice)

Matthew Lillard

Character: Shaggy Rogers (voice)

Kate Micucci

Character: Velma Dinkley (voice)

Cassandra Peterson

Character: Elvira, Mistress of the Dark (voice)

Bill Nye

Character: Bill Nye (voice)

Dwight Schultz

Character: Dr. Crane, AKA: Scarecrow (voice)

Kamali Minter

Character: Michelle (voice)

Bumper Robinson

Character: Mike (voice)

Roger Craig Smith

Character: Autopilot / Parent (voice)

Fred Tatasciore

Character: Alpha Jackal-Lantern / Federal Agent Malarkey (voice)

David Herman

Character: Sheriff, AKA: Cutler Toe (voice)