Debi Derryberry

Character: James Isaac 'Jimmy' Neutron (voice)

Christian Slater

Character: Jet Fusion (voice)

Wendie Malick

Character: Beautiful Gorgeous (voice)

Michael Clarke Duncan

Character: Commander Baker (voice)

Tim Curry

Character: Professor Calamitous (voice)

Dan Castellaneta

Character: Professor Crank (voice)

Frank Welker

Character: Goddard (voice)

Rob Paulsen

Character: Carl Wheezer / Monk / Voice / Clam (voice)

Megan Cavanagh

Character: Judy Neutron / Ms. Jingle Bells / Female Monk (voice)

Mark DeCarlo

Character: Hugh Neutron (voice)

Jeffrey Garcia

Character: Sheen Estevez (voice)

Andrea Martin

Character: Ms. Fowl (voice)

Carolyn Lawrence

Character: Cindy Vortex / Tina Sue (voice)

Crystal Scales

Character: Libby Fallfax / Ike (voice)

Kath Soucie

Character: Betty Quinlan (voice)

Corey Burton

Character: Narrator / Head Monk

Ving Rhames

Character: Chief (voice)