Zach Tyler Eisen

Character: Lucas Nickle (voice)

Julia Roberts

Character: Hova (voice)

Meryl Streep

Character: Queen (voice)

Nicolas Cage

Character: Zoc (voice)

Paul Giamatti

Character: Stan Beals (voice)

Bruce Campbell

Character: Fugax (voice)

Regina King

Character: Kreela (voice)

Lily Tomlin

Character: Mommo (voice)

Larry Miller

Character: Fred Nickle (voice)

Cheri Oteri

Character: Doreen Nickle (voice)

Ricardo Montalban

Character: Head of Council (voice)

Myles Jeffrey

Character: Steve (voice)

Creagen Dow

Character: Mullet Boy (voice)

Frank Welker

Character: Spindle / Frog / Caterpillar (voice)

Mark DeCarlo

Character: Fly (voice)

Jake T. Austin

Character: Nicky (voice)

Allison Mack

Character: Tiffany Nickle (voice)

S. Scott Bullock

Character: Glow Worm / Wasp Survivor (voice)

Paul Greenberg

Character: Sleeping Ant #1 / Head Lice #1 / Head Lice #2 / Head Lice #3 / Head Lice #4 / Brett (voice)

Rob Paulsen

Character: Beetle (voice)

Nicole Sullivan

Character: Ant #9 (voice)

Don Frye

Character: Soldier Ant (voice)

Larry Cedar

Character: Ant #8 (voice)

Paul Greenberg

Character: Sleeping Ant #1 / Head Lice #1 / Head Lice #2 (voice)

David Kaye

Character: Sleeping Ant #2 / Guard Ant #3 / Wrangler Ant (voice)

Alfred Jackson

Character: Slacker Ant (voice)

Ken Mitchroney

Character: Ant #17 / Guard Ant #2 / Wasp #8 (voice)

Denzel Whitaker

Character: Albert (voice)

Casey Masamitsu

Character: Shigeko (voice)

Bryan Fabian

Character: Fernando (voice)

Jake Goldberg

Character: Helmet Boy / Nob / Red Teammate #1 (voice)

Keith Alcorn

Character: Ant #18 / Pupa / Soldier Ant #3 (voice)

John A. Davis

Character: Ant #19 (voice)

Tom Kenny

Character: Drone Ant / Ant #2 / Ant #6 (voice)

Sarah Mensinga

Character: Mother Ant (voice)

Nika Futterman

Character: Ant #1 / Ant #7 (voice)

Scott Holst

Character: Ant #3 (voice)

Susan Silo

Character: Ant #4 (voice)

Paul Rugg

Character: Ant #5 (voice)

Candi Milo

Character: Nurse Ant #3 (voice)

Nissa Alcorn

Character: Billo (voice)

Pat Fraley

Character: Ant Council #1 (voice)

Tyler James Williams

Character: Blue Teammate #1 (voice)

Jared Goldsmith

Character: Blue Teammate #2 / Red Teammate #2 (voice)

Austin Majors

Character: Blue Teammate #3 (voice)

Jaishon Fisher

Character: Blue Teammate #4 (voice)

Aaron Michael Drozin

Character: Blue Teammate #5 (voice)

Max Burkholder

Character: Blue Teammate #6 (voice)

Sam Green

Character: Red Teammate #3 (voice)

Colin Ford

Character: Red Teammate #4 (voice)

Kendall Ryan Sanders

Character: Red Teammate #5 (voice)

Shane Baumel

Character: Red Teammate #6 (voice)

Neil Ross

Character: Wasp #1 / Wasp #5 (voice)

Bob Joles

Character: Wasp #2 (voice)

Wally Wingert

Character: Wasp #3 (voice)

Leon Morenzie

Character: Wasp #4 (voice)

Jonathan David Cook

Character: Wasp #6 (voice)

Sean Donnellan

Character: Soldier Ant #1 (voice)

Tress MacNeille

Character: Old Council Ant (voice)

Richard Green

Character: Wasp Leader (voice)

Clive Robertson

Character: Hova's Wasp (voice)

Vernee Watson-Johnson

Character: Head Nurse (voice)