Dakota Fanning

Character: Fern Arable

Julia Roberts

Character: Charlotte the Spider (voice)

Steve Buscemi

Character: Templeton the Rat (voice)

John Cleese

Character: Samuel the Sheep (voice)

Oprah Winfrey

Character: Gussy the Goose (voice)

Cedric the Entertainer

Character: Golly the Goose (voice)

Reba McEntire

Character: Betsy the Cow (voice)

Kathy Bates

Character: Bitsy the Cow (voice)

Robert Redford

Character: Ike the Horse (voice)

Thomas Haden Church

Character: Brooks the Crow (voice)

André 3000

Character: Elwyn the Crow (voice)

Dominic Scott Kay

Character: Wilbur (voice)

Sam Shepard

Character: Narrator (voice)

Abraham Benrubi

Character: Uncle the Pig (voice)

Kevin Anderson

Character: Mr. Arable

Essie Davis

Character: Mrs. Arable

Siobhan Fallon Hogan

Character: Mrs. Zuckerman

Louis Corbett

Character: Avery

Robyn Arthur

Character: Teacher

Julian O'Donnell

Character: Henry Fussy

Gary Basaraba

Character: Homer Zuckerman

Nate Mooney

Character: Lurvy

Nicholas Bell

Character: Minister

Beau Bridges

Character: Dr. Dorian

Teague Rook

Character: Well Dressed Man

Julia Zemiro

Character: Bystander

Denise Kirby

Character: New Teacher

Robert Plazek

Character: Ball Thrower

Joseph Lotesto

Character: Young Boy

Michael Roland

Character: Mayor

Don Bridges

Character: Announcer

Ian Watkin

Character: Fair Official

Joel McCrary

Character: Sheep Group (voice)

Brian Stepanek

Character: Sheep Group (voice)

Fred Tatasciore

Character: Sheep Group (voice)

Bradley White

Character: Sheep Group (voice)

Maia Kirkpatrick

Character: Joy the Baby Spider (voice)

Jennessa Rose

Character: Aranea the Baby Spider (voice)

Briana Hodge

Character: Nellie the Baby Spider (voice)

Dale Azzopardi

Character: Photographer (uncredited)

Ella Scott Lynch

Character: Girl at Fair (uncredited)