Barrie Ingham

Character: Basil of Baker Street / Bartholomew (voice)

Vincent Price

Character: Professor Ratigan (voice)

Val Bettin

Character: Dr. David Q. Dawson / Thug Guard (voice)

Susanne Pollatschek

Character: Olivia Flaversham (voice)

Candy Candido

Character: Fidget (voice)

Diana Chesney

Character: Mrs. Judson (voice)

Eve Brenner

Character: The Mouse Queen (voice)

Alan Young

Character: Mr. Flaversham (voice)

Basil Rathbone

Character: Sherlock Holmes (voice)

Ellen Fitzhugh

Character: Bar Maid (voice)

Walker Edmiston

Character: Citizen / Thug Guard (voice)

Wayne Allwine

Character: Thug Guard (voice)

Tony Anselmo

Character: Thug Guard (voice)

Frank Welker

Character: Toby the Dog / Felicia the Cat (voice) (uncredited)

Laurie Main

Character: Dr. Watson (archive sound)

Shani Wallis

Character: Lady Mouse (voice)

Melissa Manchester

Character: Miss Kitty Mouse (voice) (uncredited)